03164cam a2200289 n 450 TD1704403120191112101511.0TDMAGDIG20190501d2014 --k--ita-50----ba engSTUDY ON GAMETIC EMBRYOGENESIS VIA IN VITRO ANTHER AND ISOLATED MICROSPORE CULTURE IN FRUIT CROPSTesi di dottoratoUniversità degli Studi di Palermo2014-03-10diritti: info:eu-repo/semantics/openAccessIn relazione con info:eu-repo/semantics/altIdentifier/hdl/10447/91257tesi di dottoratoSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeUniversità degli Studi di PalermoFruit breeding is mainly based on both conventional (hybridization, mutation and selection) or biotechnological methods (somatic hybridization, genetic transformation and haploid production). The genetic improvement through the conventional methods is limited by many factors such as fruit trees long juvenile period, high heterozygosity, large size and sexual incompatibility. Haploids and doubled haploids, obtained through gametic embryogenesis have a potential use in fruit crops genetic improvement. The change of the microspores fate from the normal gametopytic pathway towards the sporophytic induction is affected by numerous factors. Genotype, medium composition and stress were considered the most important factors required to switch the pollen embryogenic development. During this doctoral course, researches have been carried out on gametic embryogenesis in different fruit crops via anther and isolated microspore culture. Particularly, for the first time, embryos were obtained by isolated microspore culture technique in Citrus spp. Morover, somatic embryogenesis callus and regeneration of plantlets were achieved via anther culture in blood sweet oranges, C. sinensis L. Osbeck, cvs. Moro, Tarocco Meli, Tarocco TDV and Tarocco S. Alfio, homozygous callus, embryos and plantlets were instead obtained from Citrus clementina Hort. ex Tan. cvs. Hernandina and Corsica. These results represent an advancement of plant breeding and propagation techniques in Citrus spp. Research has been also carried out on Olea europaea L. gametic embryogenesis, multicellular structures have been obtained from anther and isolated microspore culture, as the first step towards haploid olive embryos production. Furthermore, a preliminary experiment has been carried out on of several hazelnut (Corylus avellana L.) cultivars to obtain regeneration through anther and isolated microspore culture.Settore AGR/03- Arboricoltura Generale E Coltivazioni ArboreeTDRMOHAMED ABDELGALEL MOHAMED, AhmedGERMANA', MARIAGERMANA', MARIAITIT-FI0098http://memoria.depositolegale.it/*/http://hdl.handle.net/10447/91257http://hdl.handle.net/10447/91257http://memoria.depositolegale.it/*/http://iris.unipa.it/bitstream/10447/91257/1/tesi%20ahmed.pdfhttp://iris.unipa.it/bitstream/10447/91257/1/tesi%20ahmed.pdf CRCFTDTD