03811nam 2200265 n 450 TD18002262TDMAGDIG20190501d2016------k--ita-50----ba engACTIVE ENDOCANNABINOIDS ARE RELEASED FROM MICROGLIA IN ASSOCIATION WITH EXTRACELLULAR VESICLES TO INHIBIT GABAERGIC TRANSMISSIONTesi di dottoratoUniversità degli Studi di Milano2016-01-11diritti: info:eu-repo/semantics/openAccessIn relazione con info:eu-repo/semantics/altIdentifier/hdl/2434/346707tesi di dottoratoSettore BIO/14 - FarmacologiaUniversità degli Studi di MilanoEndocannabinoids (eCBs) are bioactive lipids which primarily influence synaptic communication within the nervous system. They are synthesized by neurons but also by microglia, especially under neuroinflammatory conditions. To exert their function, eCBs travel across the intercellular space. However, how eCBs move extracellularly remains obscure. Our recent evidence indicates that reactive microglia release extracellular vesicles (EVs), which may represent an ideal vehicle for the transport of hydrophobic eCBs. Hence, in this study we investigated whether microglial EVs carry eCBs and may influence neurotransmission. First we analyzed the eCB content of EVs and found a clear enrichment of N-arachidonoylethanolamine (AEA) in EVs relative to parental microglia. This analysis revealed higher AEA levels in EVs shed from the plasma membrane (microvesicles), compared to those which originate from the endocytic compartment (exosomes). To bioassay the activity of vesicular AEA, we used patch clamp analysis of miniature inhibitory post-synaptic currents (mIPSC) on rat hippocampal primary culture. Exposure of neurons to microvesicles (MVs) induced a significant decrease in mIPSC frequency, mimicking the well-known inhibitory action of CB1 receptor agonists. The involvement of vesicular AEA in this phenomenon was inferred from the ability of the CB1 receptor antagonist SR141716A to block the reduction of mIPSC frequency evoked by MVs. Western blot analysis showed an increase in ERK phosphorylation in neurons exposed to MVs, which was completely inhibited by SR141716A. This indicate that CB1 receptors activation by AEA-storing MVs translates into downstream signaling. Finally, the use of biotin-AEA revealed an affinity of AEA for MV membrane, indicating that AEA travels in association with MVs surface. Consistent with a surface localization of AEA, MV membranes maintain their capability to decrease mIPSC frequency. Overall, this study shows that microglial MVs carry AEA on their surface to stimulate CB1 receptors on target GABAergic neurons and demonstrates that extracellular vesicular transport of eCBs play a crucial role in the modulation of inhibitory transmission. This abstract is copyrighted © 2015 Gabrielli et al SpringerPlus 2015, 4(Suppl 1):L29 doi:10.1186/2193-1801-4-S1-L29, modified; The electronic version of this abstract is the complete one and can be found online at: http://www.springerplus.com/content/4/S1/L29 This abstract is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Settore BIO/14- FarmacologiaTDRM. GabrielliSALA, MARIAELVINAtutor: M. Sala ; co-tutor: C. Verderio ; coordinatore: A. CorsiniCORSINI, ALBERTOITIT-FI0098http://memoria.depositolegale.it/*/http://hdl.handle.net/2434/346707http://hdl.handle.net/2434/346707CFTDTD